Journal: Cells

Article Title: Response of Oral and Skin Keratinocytes to Oxidative Stress

doi: 10.3390/cells15020097

Figure Lengend Snippet: Oral keratinocytes are more tolerant to H 2 O 2 -induced oxidative injury than skin keratinocytes. ( A ) Viability of oral keratinocytes (TIGK) and skin keratinocytes (HaCaT) under different concentrations of H 2 O 2 and at different time points. The data shown is pooled from 3–4 repeated experiments. ( B ) Antioxidant effect of NAC in TIGK and HaCaT after 5 mM H 2 O 2 treatment, as assessed by viability assay. ( C ) Cytotoxicity of different H 2 O 2 concentrations in TIGK and HaCaT, as assessed by MTS assay. The data shown are representative of 3 experiments. ( D ) Antioxidant effect of NAC in TIGK and HaCaT after 5 mM H 2 O 2 treatment, as assessed by MTS assay. The dotted line indicates the level of the untreated control. ( E ) Representative bright-field images of TIGK and HaCaT treated with 5 mM H 2 O 2 . ( F ) Apoptosis was assessed by Annexin V staining. TIGK and HaCaT were treated with 5 mM H 2 O 2 for 3, 6, and 24 h. Representative images of Annexin V staining. Blue indicates DAPI nuclear staining, and green indicates Annexin V–positive cells. ( G ) Quantification of apoptosis assessment. The data shown is pooled from 3 experiments. Data are shown as mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. Two-way ANOVA followed by the Benjamini, Krieger, and Yekutieli post hoc test was used for ( A – D , G ).

Article Snippet: TIGK and HaCaT were seeded into 6-well plates and treated with the corresponding concentrations of H 2 O 2 in serum-free medium for 3 h. Following treatment, cells were stained with Annexin V-FITC according to the manufacturer’s instructions of Annexin V-FITC Apoptosis Detection Kit (Biotium, Fremont, CA, USA).

Techniques: Viability Assay, MTS Assay, Control, Staining

Journal: Cells

Article Title: Response of Oral and Skin Keratinocytes to Oxidative Stress

doi: 10.3390/cells15020097

Figure Lengend Snippet: Oral keratinocytes are more tolerant to H 2 O 2 -induced oxidative injury than skin keratinocytes. ( A ) Viability of oral keratinocytes (TIGK) and skin keratinocytes (HaCaT) under different concentrations of H 2 O 2 and at different time points. The data shown is pooled from 3–4 repeated experiments. ( B ) Antioxidant effect of NAC in TIGK and HaCaT after 5 mM H 2 O 2 treatment, as assessed by viability assay. ( C ) Cytotoxicity of different H 2 O 2 concentrations in TIGK and HaCaT, as assessed by MTS assay. The data shown are representative of 3 experiments. ( D ) Antioxidant effect of NAC in TIGK and HaCaT after 5 mM H 2 O 2 treatment, as assessed by MTS assay. The dotted line indicates the level of the untreated control. ( E ) Representative bright-field images of TIGK and HaCaT treated with 5 mM H 2 O 2 . ( F ) Apoptosis was assessed by Annexin V staining. TIGK and HaCaT were treated with 5 mM H 2 O 2 for 3, 6, and 24 h. Representative images of Annexin V staining. Blue indicates DAPI nuclear staining, and green indicates Annexin V–positive cells. ( G ) Quantification of apoptosis assessment. The data shown is pooled from 3 experiments. Data are shown as mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. Two-way ANOVA followed by the Benjamini, Krieger, and Yekutieli post hoc test was used for ( A – D , G ).

Article Snippet: TIGK and HaCaT were seeded into 6-well plates and treated with the corresponding concentrations of H 2 O 2 in serum-free medium for 3 h. Following treatment, cells were stained with Annexin V-FITC according to the manufacturer’s instructions of Annexin V-FITC Apoptosis Detection Kit (Biotium, Fremont, CA, USA).

Techniques: Viability Assay, MTS Assay, Control, Staining